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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
02/07/2021 |
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Actualizado : |
02/07/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
DE LORENZO, C.; BOABAID, F. M.; OLIVEIRA, L.G.S.; BIANCHI, M. V.; FÉLIX, M.L.; ARMÚA-FERNÁNDEZ, M.T.; SOARES, J.F.; VENZAL, J.M.; SONNE, L. |
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Afiliación : |
CÍNTIA DE LORENZO, Setor de Patologia Veterinária, Departamento de Patologia Clínica Veterinária, Faculdade de Veterinária (FAVET), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil; FABIANA MARQUES BOABAID, Polo de Desarrollo Universitario del Instituto Superior de la Carne, Sede Tacuarembó, Centro Universitario Regional (CENUR) Noreste, Universidad de la República, Tacuarembó, Uruguay; LUIZ GUSTAVO SCHNEIDER DE OLIVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; MATHEUS VIEZZER BIANCHI, Setor de Patologia Veterinária, Departamento de Patologia Clínica Veterinária, Faculdade de Veterinária (FAVET), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil; MARÍA L. FÉLIX, Laboratorio de Vectores y enfermedades transmitidas, Departamento de Ciencias Biológicas, CENUR Litoral Norte – Salto, Universidad de la República, Salto, Uruguay; MARÍA T. ARMÚA-FERNÁNDEZ, Laboratorio de Vectores y enfermedades transmitidas, Departamento de Ciencias Biológicas, CENUR Litoral Norte – Salto, Universidad de la República, Salto, Uruguay; JOAO FABIO SOARES, Laboratório de Protozoologia e Rickettsioses Vetoriais (ProtozooVet), Departamento de Patologia Clínica Veterinária, FAVET, UFRGS, Porto Alegre, Rio Grande do Sul, RS, Brazil; JOSÉ M. VENZAL, Laboratório de Protozoologia e Rickettsioses Vetoriais (ProtozooVet), Departamento de Patologia Clínica Veterinária, FAVET, UFRGS, Porto Alegre, Rio Grande do Sul, RS, Brazil; LUCIANA SONNE, Setor de Patologia Veterinária, Departamento de Patologia Clínica Veterinária, Faculdade de Veterinária (FAVET), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil. |
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Título : |
Rangelia vitalii in free-living crab-eating foxes (Cerdocyon thous) in Uruguay. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Ticks and Tick-borne Diseases, 2021, Volume 12, Issue 5, Article number 101765. Doi: https://doi.org/10.1016/j.ttbdis.2021.101765 |
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ISSN : |
1877-959X |
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DOI : |
10.1016/j.ttbdis.2021.101765 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 25 November 2020; Received in revised form 1 May 2021; Accepted 13 May 2021; Available online 4 June 2021.
This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico ( CNPq ) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior ( CAPES 001 ).
Corresponding author: Soares, J.F.; Laboratório de Protozoologia e Rickettsioses Vetoriais (ProtozooVet), UFRGS, Avenida Bento Gonçalves, Porto Alegre, Rio Grande do Sul, Brazil; email:joao.soares@ufrgs.br |
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Contenido : |
ABSTRACT - Rangelia vitalii is a protozoan parasite that causes a hemorrhagic and hemolytic disease in dogs known as rangeliosis. Current reports of the disease are concentrated in the southern and southeastern regions of Brazil, as well as in Uruguay, Argentina, and Paraguay, and mainly concern domestic dogs. South American wild canids, such as the crab-eating fox (Cerdocyon thous), the pampas fox (Lycalopex gymnocercus), and the maned wolf (Chrysocyon brachyurus) may also be affected, although existing reports are restricted to Brazil. The present study aimed to detect R. vitalii parasitism in the Uruguayan wild fox population. DNA extracted from the blood and/or spleen samples of road-killed C. thous and L. gymnocercus found in northern Uruguay were subjected to polymerase chain reaction (PCR) to amplify a 551-bp fragment of the Rangelia 18S rRNA gene. A total of 62 wild canids, including 38 C. thous and 24L. gymnocercus, were analyzed. Five crab-eating fox samples (13.2%) were positive for R. vitalii, with 99.5?100% identity between the sequences. All samples from pampas fox tested negative for R. vitalii. When compared with the R. vitalii sequences available in GenBank, a similarity of 98.9?100% was revealed. Molecular analysis results suggest that R. vitalii is circulating in the crab-eating fox population in Uruguay; however, its veterinary relevance for these foxes remains unknown. © 2021 |
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Palabras claves : |
DNA; Dog; Hemoparasites; Rangelia vitalii; Tick-borne disease; Wild canids. |
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Asunto categoría : |
A50 Investigación agraria |
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Marc : |
LEADER 02910naa a2200325 a 4500
001 1062216
005 2021-07-02
008 2021 bl uuuu u00u1 u #d
022 $a1877-959X
024 7 $a10.1016/j.ttbdis.2021.101765$2DOI
100 1 $aDE LORENZO, C.
245 $aRangelia vitalii in free-living crab-eating foxes (Cerdocyon thous) in Uruguay.$h[electronic resource]
260 $c2021
500 $aArticle history: Received 25 November 2020; Received in revised form 1 May 2021; Accepted 13 May 2021; Available online 4 June 2021. This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico ( CNPq ) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior ( CAPES 001 ). Corresponding author: Soares, J.F.; Laboratório de Protozoologia e Rickettsioses Vetoriais (ProtozooVet), UFRGS, Avenida Bento Gonçalves, Porto Alegre, Rio Grande do Sul, Brazil; email:joao.soares@ufrgs.br
520 $aABSTRACT - Rangelia vitalii is a protozoan parasite that causes a hemorrhagic and hemolytic disease in dogs known as rangeliosis. Current reports of the disease are concentrated in the southern and southeastern regions of Brazil, as well as in Uruguay, Argentina, and Paraguay, and mainly concern domestic dogs. South American wild canids, such as the crab-eating fox (Cerdocyon thous), the pampas fox (Lycalopex gymnocercus), and the maned wolf (Chrysocyon brachyurus) may also be affected, although existing reports are restricted to Brazil. The present study aimed to detect R. vitalii parasitism in the Uruguayan wild fox population. DNA extracted from the blood and/or spleen samples of road-killed C. thous and L. gymnocercus found in northern Uruguay were subjected to polymerase chain reaction (PCR) to amplify a 551-bp fragment of the Rangelia 18S rRNA gene. A total of 62 wild canids, including 38 C. thous and 24L. gymnocercus, were analyzed. Five crab-eating fox samples (13.2%) were positive for R. vitalii, with 99.5?100% identity between the sequences. All samples from pampas fox tested negative for R. vitalii. When compared with the R. vitalii sequences available in GenBank, a similarity of 98.9?100% was revealed. Molecular analysis results suggest that R. vitalii is circulating in the crab-eating fox population in Uruguay; however, its veterinary relevance for these foxes remains unknown. © 2021
653 $aDNA
653 $aDog
653 $aHemoparasites
653 $aRangelia vitalii
653 $aTick-borne disease
653 $aWild canids
700 1 $aBOABAID, F. M.
700 1 $aOLIVEIRA, L.G.S.
700 1 $aBIANCHI, M. V.
700 1 $aFÉLIX, M.L.
700 1 $aARMÚA-FERNÁNDEZ, M.T.
700 1 $aSOARES, J.F.
700 1 $aVENZAL, J.M.
700 1 $aSONNE, L.
773 $tTicks and Tick-borne Diseases, 2021, Volume 12, Issue 5, Article number 101765. Doi: https://doi.org/10.1016/j.ttbdis.2021.101765
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha actual : |
13/04/2022 |
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Actualizado : |
17/05/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
PEREIRA MORA, L.; TERRA, J.A.; FERNÁNDEZ SCAVINO, A. |
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Afiliación : |
LUCIANA PEREIRA MORA, Area Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay.; JOSÉ ALFREDO TERRA FERNÁNDEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA FERNÁNDEZ SCAVINO, Area Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay. |
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Título : |
Methanogenic community linked to organic acids fermentation from root exudates are affected by rice intensification in rotational soil systems. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Applied Soil Ecology, 2022, volume 176, artículo 104498. OPEN ACCESS. doi: https://doi.org/10.1016/j.apsoil.2022.104498 |
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Páginas : |
9 p. |
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DOI : |
10.1016/j.apsoil.2022.104498 |
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Idioma : |
Inglés |
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Notas : |
History Article: Received 30 December 2021; Received in revised form 1 April 2022; Accepted 4 April 2022; Available online 13 April 2022.
Corresponding author: afernand@fq.edu.uy (A. Fernandez-Scavino). |
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Contenido : |
Rice paddy soils are an important biogenic source of methane, a relevant greenhouse gas. Rice rotation with upland crops and pastures affects hydrolytic and fermentative bacteria and methanogenic archaea by sequential exposition to oxic and anoxic conditions. There are several ways to reduce methane emissions from rice paddy soils. Here we examine the effect of intensification in rice rotations with upland crops upon the microbial community structure and methanogenesis when dicarboxylic acids exudated by rice root were amended in microcosms assays. Soils under rotations rice-pasture, rice-soybean and continuous rice, with the last two more intensive systems recently installed in the field experiment, were sampled. Amplicon sequencing of 16S rRNA gene analysis showed that the rotation system was a main driver of the microbial community structure, explaining 33%, 38% and 55% of the differences in the structure of Bacteria, Archaea and methanogens,
respectively. The density of mcrA gene copies was significantly higher in continuous rice soil than in other soils, and the relative abundance of methanogenic archaea was 33% for rice monoculture, whereas represented 15% and 14% for the rice-pasture and rice-soybean soils, respectively. The incubation of soils with tartrate and
succinate confirmed functional differences among soils since rice monoculture showed similar or higher methane production and significantly less acetate and propionate accumulation than other soils. The T-RFLP analysis of mcrA gene in soils and incubated soils revealed that Rice Cluster I increased after incubation in all rotational soils, whereas Methanosarcinaceae and Methanobacteriaceae remained relevant after incubation. Furthermore, a more stable community of methanogenic archaea was established in soil under continuous rice, since T-RF profiles of soils and incubated soils with dicarboxylic acids grouped together with 62% similarity, while communities from other rotational soils experimented a great shift after incubation with these substrates. Altogether, these results showed that the conversion of rice-pasture rotational fields to a more intensive rice crop production strongly impacts the methanogenic community structure and their potential for methane emission. MenosRice paddy soils are an important biogenic source of methane, a relevant greenhouse gas. Rice rotation with upland crops and pastures affects hydrolytic and fermentative bacteria and methanogenic archaea by sequential exposition to oxic and anoxic conditions. There are several ways to reduce methane emissions from rice paddy soils. Here we examine the effect of intensification in rice rotations with upland crops upon the microbial community structure and methanogenesis when dicarboxylic acids exudated by rice root were amended in microcosms assays. Soils under rotations rice-pasture, rice-soybean and continuous rice, with the last two more intensive systems recently installed in the field experiment, were sampled. Amplicon sequencing of 16S rRNA gene analysis showed that the rotation system was a main driver of the microbial community structure, explaining 33%, 38% and 55% of the differences in the structure of Bacteria, Archaea and methanogens,
respectively. The density of mcrA gene copies was significantly higher in continuous rice soil than in other soils, and the relative abundance of methanogenic archaea was 33% for rice monoculture, whereas represented 15% and 14% for the rice-pasture and rice-soybean soils, respectively. The incubation of soils with tartrate and
succinate confirmed functional differences among soils since rice monoculture showed similar or higher methane production and significantly less acetate and propionate accumulation than other soils. The T-RFLP... Presentar Todo |
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Palabras claves : |
CROP ROTATION; CROP ROTATION SYSTEMS; METHANOGENS; RICE INTENSIFICATION; ROOT EXUDATION. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 03285naa a2200241 a 4500
001 1063031
005 2022-05-17
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1016/j.apsoil.2022.104498$2DOI
100 1 $aPEREIRA MORA, L.
245 $aMethanogenic community linked to organic acids fermentation from root exudates are affected by rice intensification in rotational soil systems.
260 $c2022
300 $a9 p.
500 $aHistory Article: Received 30 December 2021; Received in revised form 1 April 2022; Accepted 4 April 2022; Available online 13 April 2022. Corresponding author: afernand@fq.edu.uy (A. Fernandez-Scavino).
520 $aRice paddy soils are an important biogenic source of methane, a relevant greenhouse gas. Rice rotation with upland crops and pastures affects hydrolytic and fermentative bacteria and methanogenic archaea by sequential exposition to oxic and anoxic conditions. There are several ways to reduce methane emissions from rice paddy soils. Here we examine the effect of intensification in rice rotations with upland crops upon the microbial community structure and methanogenesis when dicarboxylic acids exudated by rice root were amended in microcosms assays. Soils under rotations rice-pasture, rice-soybean and continuous rice, with the last two more intensive systems recently installed in the field experiment, were sampled. Amplicon sequencing of 16S rRNA gene analysis showed that the rotation system was a main driver of the microbial community structure, explaining 33%, 38% and 55% of the differences in the structure of Bacteria, Archaea and methanogens, respectively. The density of mcrA gene copies was significantly higher in continuous rice soil than in other soils, and the relative abundance of methanogenic archaea was 33% for rice monoculture, whereas represented 15% and 14% for the rice-pasture and rice-soybean soils, respectively. The incubation of soils with tartrate and succinate confirmed functional differences among soils since rice monoculture showed similar or higher methane production and significantly less acetate and propionate accumulation than other soils. The T-RFLP analysis of mcrA gene in soils and incubated soils revealed that Rice Cluster I increased after incubation in all rotational soils, whereas Methanosarcinaceae and Methanobacteriaceae remained relevant after incubation. Furthermore, a more stable community of methanogenic archaea was established in soil under continuous rice, since T-RF profiles of soils and incubated soils with dicarboxylic acids grouped together with 62% similarity, while communities from other rotational soils experimented a great shift after incubation with these substrates. Altogether, these results showed that the conversion of rice-pasture rotational fields to a more intensive rice crop production strongly impacts the methanogenic community structure and their potential for methane emission.
653 $aCROP ROTATION
653 $aCROP ROTATION SYSTEMS
653 $aMETHANOGENS
653 $aRICE INTENSIFICATION
653 $aROOT EXUDATION
700 1 $aTERRA, J.A.
700 1 $aFERNÁNDEZ SCAVINO, A.
773 $tApplied Soil Ecology, 2022, volume 176, artículo 104498. OPEN ACCESS. doi: https://doi.org/10.1016/j.apsoil.2022.104498
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